More than 7,000 fungal cultures have been acquired and are in liquid nitrogen storage. Thus far, about 3,500 cultures have been grown in a variety of media. Fungal ferments consisting of broth and cells, typically 800 MLS, are homogenized after the addition of 10% V/V methanol, then, in a separatory funnel, an equal volume of dichloromethane is added. After agitation and separation of the organic layer, the cell debris is removed by filtration and the broth is lyophilized, thus giving both organic soluble and water soluble extracts for screening. Extracts of fungi selected for regrow at a larger scale, up to 80 L, are also prepared.
This graph shows the number of Fungus extracted per year between 1987-2011. 922 Fungal extracts were done in 2011.