MAIN HEADING

 

NATURAL PRODUCTS SUPPORT GROUP

 

 

Recent publications arising from the work of this group includes:  

 

Paul Klausmeyer, Thomas G. McCloud, Jalpa Shah, Melinda Hollingshead, Jerry Collins, David J. Newman.  New Antitumor Lead Candidate Derived from in vivo Screening of an Organic Extract from Garcinia laterifolia.  Poster presented at the American Society of Pharmacognosy 51st Annual Meeting, St. Petersburg, FL, 10-14 July, 2010.

Thomas G. McCloud. High Throughput Extraction of Plant, Marine and Fungal Specimens for Preservation of Biologically Active Molecules. Molecules  2010, 15(7), 4526-4563 24 June 20 2010

Klausmeyer P,  McCloud TG, Zinger N, Hollingshead M, Collins JM, Newman DJ:  Cytotoxic Metabolites from a Marine Sponge of the Order Axinellida.   Poster presented at The American Society of Pharmacognosy 50th Annual Meeting, Honolulu, HI,  27 Jun - 1 Jul, 2009

Harris MJ ,  McCloud TG. :  Nondestructive Elimination of Saponins from Crude Extracts. Poster presented at The American Society of Pharmacognosy 50th Annual Meeting, Honolulu, HI,  27 Jun - 1 Jul, 2009.

Klausmeyer P,  Hayavi S, McCloud TG, Parchment RE, Newman D. :  Development of an Ultra-sensitive UPLC/TOFMS Assay for Drug Recovery from Plasma in Preclinical Investigations.   Poster presented at Ft. Detrick Spring Research Festival, 5 May 2009.

Klausmeyer P, Zhou Q, Scudiero DA, Uranchimeg, B, Melillo G: Cardellina JHII, Shoemaker RH, Chang C-j, McCloud TG.  Cytotoxic and HIF-Inhibitory Compounds from Crossosoma bigelovii  Journal of Natural Products 2009, 72(5), 805-812.

 Klausmeyer P , Howard OMZ, Shipley SM, McCloud TG:  An Inhibitor of CCL2-Induced Chemotaxis from the Fungus Leptoxyphium sp.   Journal of Natural Products electronic publication 28 Jul 2009

Waybright TJ, Britt JR, McCloud TG:  Overcoming Problems of Compound Storage in DMSO: Solvent and Process Alternatives  Journal of Biomolecular Screening  14 (6): 708 (2009)

 Klausmeyer P:   Cis-stilbenes from Macaranga schweinfurthii.  Oral presentation to the schweinfurthin research group, NCI-Frederick, 12 Dec 2008. 

Kim H, Cardellina JH II, Akee R, Champoux JJ, Stivers JT: Arylstibonic Acids: Novel Inhibitors and Activators of Human Topoisomerase IB. Bioorganic Chemistry 36: 190-197, 2008

Seiple LA, Cardellina JH II, Akee R, Stivers JT: Potent Inhibition of Human Apurinic/Apyrimidinic Endonuclease 1 by Arylstibonic Acids: Molecular Pharmacology 73: 669-677, 2008

Klausmeyer P, McCloud TG, Uranchimeg B, Melillo G, Scudiero DA, Cardellina JH, Shoemaker RH:  Separation and SAR Study of HIF-1α Inhibitory Tubulosines from Alangium cf. longiflorum. Planta Medica 74: 258-263, 2008

Klausmeyer P, Cardellina JH II, Scudiero DA, Shoemaker RH, and McCloud TG: HIF-1α Active Components of the Unstudied Plant Crossosoma bigelovii.  Presented at the 7th Annual Oxford Conference on the Science of   Botanicals and American Society of Pharmacognosy 4th Interim Meeting,  April 12-17, 2008, Oxford, MS

Klausmeyer P, McCloud TG, Uranchimeg B, Melillo G, Scudiero, DA, Cardellina JH II, Shoemaker RH:  HIF-1a SAR Study of Tubulosines Isolated from Alangium cf. longiflorum - poster presented at the American Society of Pharmacognosy 48th Annual Meeting, Portland, ME, July 14-18, 2007.

SM Shipley, AL Barr, SJ Graf, RP Collins, TG McCloud, and DJ Newman: Development of a Process for the Production of the Anticancer Lead Compound Pleurotin by Fermentation of Hohenbuehelia atrocaerulea  Jol. Industrial Microbiology and Biotechnology (2006) 33:463-468

 Paul Klausmeyer, Thomas G. McCloud,  Giovanni Melillo, Dominic A. Scudiero, John H. Cardellina II, and Robert H. Shoemaker:  Identification of a New Natural Camptothecin Analog in Targeted Screening for HIF-1α Inhibitors     Planta Medica 73: 49-52, 2007.

Waybright TJ, Britt, JR, and McCloud TG.  Oral Presentation:   Solving the problems with DMSO in Biological Testing.  American Society of Pharmacognosy 47th annual meeting, Arlington, VA,  August 5-9, 2006.

Klausmeyer, P*, McCloud, TG, Scudiero, DA, Shoemaker, RH, and Cardellina, JH, II. :  Poster Presentation: Bisbenzylisoquinoline Alkaloids from Gyrocarpus jacquini Restore C/EBPα Expression in an in vitro Molecular Screen.   American Society of Pharmacognosy 47th Annual Meeting, Arlington, VA,  August 5-9, 2006.

 Paul Klausmeyer, Thomas G. McCloud, Kenneth D. Tucker, John H. Cardellina II, and Robert H. Shoemaker: Aspirochlorine Class Compounds from Aspergillus flavus Inhibit Azole-Resistant Candida albicans     Journal of Natural Products, 2005, 68, 1300-1302.

Waybright TJ, McCloud TG: Overcoming the problems of long term storage of compounds in DMSO of Synthetic and Naturally Derived Compounds. Poster Presented at the American Society of Pharmacognosy 46th annual meeting, Corvallis, OR, July 23-27, 2005.

Klausmeyer P, McCloud TG, Tucker KD, Cardellina, JH II, & Shoemaker RH: Aspirochlorine Class Compounds from Aspergillus flavus Inhibit Azole-Resistant Candida albicans.  Poster Presented at the American Society of Pharmacognosy 46th annual meeting, Corvallis, OR, July 23-27, 2005.

McCloud, TG:  High Throughput Extraction of Plants for Preservation of Biologically Active Molecules.  Poster Presented at the American Chemical Society, Green Chemistry Symposium, 29 August 2005, Washington DC.

Klausmeyer P, Chmurny GN, McCloud TG, Tucker KD, Shoemaker RH: Novel antimicrobial indolizinium alkaloid from Aniba panurensis. Journal of Natural Products, 2004, 67, 1732-1735.

Paul Klausmeyer, Gwendolyn N. Chmurny, Thomas G. McCloud,* Kenneth D. Tucker, and Robert H. Shoemaker: A Novel Antimicrobial Alkaloidfrom Aniba panurensis . Poster Presented at the 2004 International Congress on Natural Product Research, A joint meeting of the ASP, AFERP, GA and the PSE July 31-August 4, 2004 Phoenix, AZ.

M. Shipley,  A.L. Barr, S.J. Graf, R.P. Collins, T.G. McCloud, and D.J. Newman: Development of a Process for the Production of the Anticancer Lead Compound Pleurotin by Fermentation of Hohenbuehelia atrocaerulea.   Poster Presented at the 2004 International Congress on Natural Products Research, A joint meeting of the ASP, AFERP, GA and the PSE  July 31-August 4, 2004 Phoenix, AZ; Podium Presentation at the July 2004 Society of Microbiology meeting, Anaheim, CA.

Timothy J. Waybright and Thomas G. McCloud: Overcoming the Problems Associated with Long-Term Storage of Compounds in DMSO.  Presented at 2004 NCI-Frederick/Ft. Detrick Spring Research Festival, May 12-13, 2004 Ft. Detrick, Maryland  [Please Note: this is the Link to NSC Numbers]

Timothy J. Waybright and Thomas G. McCloud: pH Zone-Refining Countercurrent Chromatography for Resolution of Tropane Alkaloids and Applications for Preparative Scale Separations. Presented at 44th Annual Meeting of the American Society of Pharmacognosy “The Changing Face of Natural Products Chemistry” July 12-16, 2003 Chapel Hill, North Carolina

Rhone K. Akee, Suzanne M. Shipley, Sally J. Graf, David J. Newman and Thomas G. McCloud: Fermentation and Purification Methods for the Production of Leptomycin B and Related Analogs from Streptomyces SP.  Presented at 44th Annual Meeting of the American Society of Pharmacognosy “The Changing Face of Natural Products Chemistry” July 12-16, 2003 Chapel Hill, North Carolina

T. Luke Simmons and Thomas G. McCloud: Analysis of Stibonic Acids by Ion Exchange Chromatography with ESI-MS/Photodiode Array Detection.  Journal of Liquid Chromatography & Related Technologies 26(13) 2041-2051, 2003

Thomas G. McCloud, Barry R. O’Keefe, and Gordon Cragg: Bioactive Molecules From Water Extracts: 15 Years Experience at FCRDC. Presented at 44th Annual Meeting of the American Society of Pharmacognosy “The Changing Face of Natural Products Chemistry” July 12-16, 2003 Chapel Hill, North Carolina

Andrew G. Stephen, Karen W. Worthy, Eric Towler, Judy A. Mikovits, Shizuko Sei, Paula Roberts, Quan-en Yang, Rhone K. Akee, Paul Klausmeyer, Thomas G. McCloud, Lou Henderson, Alan Rein, David G. Covell, Micheal Currens, Robert H. Shoemaker, and Robert J. Fisher: Identification of HIV-1 Nucleocapsid Protein: Nucleic Acid Antagonists with Cellular Anti-HIV Activity. Biochemical and Biophysical Research Communications 296: 1228-1237, 2002

Paul Klausmeyer, T. Luke Simmons, Thomas G. McCloudComputer and Database Tools for the Identification of Novel Lead Compounds in Natural Products Extracts. Presented at the 43rd Annual Meeting of the American Society of Pharmacognosy and 3rd Monroe Wall Symposium July 27-31, 2002 Hyatt Regency Hotel, New Brunswick, New Jersey

Rhone K. Akee, Robert H. Shoemaker, and Thomas G. McCloud: Synthesis of  Fluorescein and Gallein and Gallein Derivatives: Physico-Chemical Properties.  Presented at the 43rd Annual Meeting of the American Society of Pharmacognosy and 3rd Monroe Wall Symposium July 27-31, 2002 Hyatt Regency Hotel, New Brunswick, New Jersey

Rhone K. Akee, Sally J. Graf, Thomas G. McCloud: The Diverse Toxins of  Aspergillus fumigatus MDH37025. Presented at the 43rd Annual Meeting of the American Society of Pharmacognosy and 3rd Monroe Wall Symposium July 27-31, 2002 Hyatt Regency Hotel, New Brunswick, New Jersey

Timothy J. Waybright, Thomas G. McCloud:  Isoelectric Focusing: A Novel Approach for Isolation of Small, Water-Soluble Molecules From Natural Products Extracts. Presented at the 43rd Annual Meeting of the American Society of Pharmacognosy and 3rd Monroe Wall Symposium July 27-31, 2002 Hyatt Regency Hotel, New Brunswick, New Jersey

McCloud, T.G., Klausmeyer, P, Newman, D.J:  Dereplication of Penicillium Bioactives Using Multidector HPLC and Bioactivity Profiles .  Presented at the American Society of Pharmacognosy Annual Meeting July 22-26, 2000, Seattle, WA. 

McCloud, T.G., Burnette, A. , Collins, R., Graf, S., Zhu, J., Reeb, S., Testerman, R., Wang, S. D., Newman, D. J: Development of a Process for the Production of the Anticancer Lead compound Wortmannin by the fermentation of Pencillium duclauxii.  Presented at the Third Conference on Recent Advances in Fermentation Technology, Sarasota, FL November 13-16, 1999.

McCloud, T.G., Britt, J.R., Cartner, L.K., Pearl, K.C., Majadly, F.D., Muschik, G.M., Klueh, P.A., Cragg, G.M., Thomas, D.W., Jato, J.G., Simon, J.E.: Quantitation of the Anti-HIV Alkaloids Michellamine A & B by HPLC.  Phytochemical Analysis 8: 120 - 123, 1997.

Simon, J.E.,Jato, J.C., Thomas, D.W., Symonds, P., Alkire, B.H., McCloud, T.G., C., Cragg, G.M., Morales, M.R., Foster, P.F., Miller, J.S., Gereau, R.E.: (1996) “Investigations on Ancistrocladus korupensis: A Promising Source of Michellamine B from Cameroon”, J. Soc. Economic Botany 

Beutler,J.A., Alvarado-Lindner, A.B. and McCloud, T.G.:  Further studies on phorbol ester bioactivity in the Euphorbiaceae.  Annals of the Missouri Botanical Garden 83:530-533, 1996.

McCloud, T.G., Klueh, P.A., Pearl, K.C., Cartner, L.K.,  Muschik, G.M., Poole, K.K. : Isolation of illudin S from the mature basidiocarp of Omphalotus illudens, and isolation of illudins S and M from the fermentation broth of Omphalotusolearis. Natural Product Letters 9: 87-95, 1996. 

McCloud, T.G., Burns, M.P., Majadly, F.D., Muschik, G.M., Miller, D.A., Poole, K.K., Roach, J.M., Ross, J.T., Lebherz,W.B., III:  Production of Brefeldin-A.  Jol. of  Industrial Microbiology 15: 5-9, 1995.

Chan, K.C., Majadly, F., McCloud, T.G., Muschik, G.M., Issaq, H.J. and Snader, K.M.: Micellar electrokinetic chromatography of the anti-human immunodeficiency virus (HIV) agent michellamine B with absorption and laser-induced  fluorescence detection.  Electrophoresis 15: 1310-1315, 1994.

Beutler, J.A., McKee, T.C., Fuller, R.W., Tischler, M., Cardellina, J.H.,II, McCloud, T.G., Snader, K.M. and Boyd, M.R.:  Frequent occurrence of HIV-inhibitory sulfated polysaccharides in marine invertebrates.  Antiviral Chem.& Chemotherapy 3: 167-172, 1993.

Schaufelberger, D.E., McCloud, T.G. and Beutler, J.A.: Laser-light-scattering detection for high-speed countercurrent chromatography. J. Chromatogr. 538(1): 87-90, 1991.

Beutler, J.A., Alvarado, A.B., Schaufelberger, D.E., Andrews, P. and McCloud, T.G.: Dereplication of phorbol bioactives: Lyngbya majuscula and Croton cuneatus.  J. Nat.Prod. 53: 867-874, 1990.       

Witherup, K.M., Look, S.A., Stasko, M.W., McCloud, T.G., Issaq, H.J., Muschik, G.M.:  High Performance liquid chromatographic separation of taxol and related compounds from Taxus brevifolia.  J. Liq. Chromatogr. 12(11), 2117-32,1989.  

Stasko, M.W., Witherup, K.M., Ghiorzi, T.J.,  McCloud, T.G., Look, S., Muschik, G.M., Issaq, H.J.:  Multimodal thin layer chromatographic separation of taxol and related compounds from Taxus brevifolia. J. Liq. Chromatogr. 12(11), 2133-  43, 1989. 

Beutler, J.A., Alvarado, A.B., McCloud, T.G. and Cragg,G.M.: Distribution of phorbol ester bioactivity in the Euphorbiaceae. Phytotherapy Res. 3: 188-192, 1989.  


 

Click the link to go back: http://npsg.ncifcrf.gov/